弥漫大B细胞淋巴瘤中TMS1基因启动子甲基化异常的研究

Promoter methylation of TMS1 gene in diffuse large B cell lymphoma

目的:研究弥漫大B细胞淋巴瘤中甲基化诱导沉默基因1(TMS1)基因启动子甲基化及其与弥漫大B细胞淋巴瘤不同细胞起源类型之间的相关性。方法:应用免疫组织化学方法检测40例弥漫大B细胞淋巴瘤石蜡包埋组织中CD10、Bcl6和MUM1的表达,并进一步区分生发中心与非生发中心B细胞起源类型;甲基化特异性PCR技术检测TMS1基因启动子甲基化状态。结果:弥漫大B细胞淋巴瘤40例中,生发中心B细胞起源16例,非生发中心B细胞起源24例。弥漫大B细胞淋巴瘤14例中存在TSM1/ASC基因异常甲基化,其中生发中心B细胞起源2例(12.5%),非生发中心B细胞起源12例(50.0%),两者阳性率比较差异有统计学意义(P<0.01)。结论:TSM1基因启动子甲基化在弥漫大B细胞淋巴瘤中常见,且对非生发中心B细胞起源发生进展更为重要。

Objective：To investigate promoter methylation of TMS1 gene in diffuse large B cell lymphoma（DLBCL） and its corre-lation with different cell origin types.Methods：Expression of CD10,Bcl6 and MUM1 was detected by immunohistochemitry on paraf-fin-embedded tissues of 40 cases of DLBCL.Germinal center B-cell like（GCB） or non-GCB type was identified by using an algorithm based on the three above antibodies.Methylation specific PCR was also employed to detect methylation status of TMS1 gene promoter in all the 40 cases.Results：Of the 40 DLBCL cases,16 were labeled as GCB-DLBCL,and 24 as non-GCB-DLBCL.Promoter methy-lation of TMS1 gene was detected in 14 of the 40 DLBCL cases.TMS1 promoter methylation was observed more often in non-GCB DL-BCL than in GCB-DLBCL（P〈0.01）.Conclusions：Promoter methylation of TMS1 gene is a frequent event in DLBCL.The role for such aberrant methylation may be particularly important for DLBCL of the non-GCB type.