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接枝层厚度对聚(N-异丙基丙烯酰胺)改性表面与蛋白质相互作用的影响

Influence of the Grafted Layer Thickness on the Interaction between Poly(N-isopropylacrylamide) Modified Surfaces and Proteins
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摘要 采用表面引发原子转移自由基聚合(ATRP)的方法制备了聚(N-异丙基丙烯酰胺)(PNIPAAm)改性表面,通过改变聚合时间进而调控表面接枝层厚度。水接触角和蛋白质吸附测试发现PNIPAAm改性表面的浸润性和对蛋白质吸附都具有一定的温度响应性,且随着PNIPAAm接枝层厚度的增加,该温度响应性有一定程度的增强。同时,纤维蛋白原和溶菌酶的吸附测试表明PNIPAAm改性表面对尺寸不同的蛋白质具有不同的温度响应性。 The poly（N-isopropylacrylamide） （PNIPAAm） brushes are prepared via surface-initiated atom transfer radical polymerization （SI-ATRP） on initiator-immobilized silicon surfaces. The thickness of grafted PNIPAAm layer is modulated by adjusting polymerization time. The PNIPAAm modified surface exhibits thermo-re- sponsivity to both wettability and protein adsorption in some extent and the thermo-responsivity is enhanced as the thickness of PNIPAAm layer increases. Moreover, the adsorption results of two proteins（fibrinogen and lysozyme） with different sizes indicate that the PNIPAAm-modified surface exhibits a size-sensitive property of protein adsorption.
作者 于谦 张燕霞 徐亚骏 陈红
机构地区 武汉理工大学材料科学与工程学院 苏州大学材料与化学化工学部
出处 《材料导报》 EI CAS CSCD 北大核心 2010年第22期25-28,共4页 Materials Reports
基金 国家自然科学基金(20974086)
关键词 聚(N-异丙基丙烯酰胺) 温度响应性 蛋白质吸附 厚度 poly（N-isopropylacrylamide）, thermo-responsivity, protein adsorption, thickness
分类号 O633.22 [理学—高分子化学]
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参考文献15

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材料导报
2010年 第22期

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