冬虫夏草菌种的制备及液体培养基的优化研究

Study on Preparation and Optimization for Liquid Culture Media of Cordyceps Sinensis

通过改变传统的冬虫夏草菌种制备的方法,利用其菌索来制备,并对其菌丝体的液体培养基的配方进行优化。结果表明,冬虫夏草生长最适宜液体培养基的配方为葡萄糖7.5%、蔗糖7.5%、蛋白胨0.12%、酵母粉0.375%、KH_2PO_40.15%、MgSO_4·7H_2O 0.075%、VB_10.015%、pH自然,20℃条件下培养菌丝体192 h,菌丝的量达到4.242g/L,并且在以后的二、三级菌种培养过程中,菌丝的性状表现优良,生长速度较快。

Different from the traditional Cordyceps Sinensis preparation methods, the Cordyceps Sinensis rhizomorph has been used to prepare and optimize the liquid culture media of cordyceps sinensis mycelia. Results show that the optimal medium for the mycelia has 7.5% glucose, 7.5% sucrose, 0.12% peptone, 0.375% yeast powder, 0.15 % KH2PO4 , 0.075 % MgSO4＊ 7H2O, 0.015% VB1 and natural pH. Under the cultural condition of 20 ℃ and 192 h, the mycelia biomass may reach 4.242g/L, and in the next Grade 2 and Grade 3 Culturing processes, the hyphae has excellent characters and grows faster.