高效液相色谱法同时测定黄龙胶囊中4种成分的含量

Simultaneous determination 4 contents of anthraquinone in the Huanglong capsule by HPLC

目的建立同时测定黄龙胶囊中4种蒽醌成分含量的方法,提高已有标准。方法采用Aglient XDBC18柱(250 mm×4.6 mm,5μm),甲醇-0.05%磷酸(86:14)为流动相,流速:1.0 ml/min,柱温:35℃,检测波长:254 nm。结果芦荟大黄素在16.28～162.8μg/ml(r=0.9994),大黄酸在10.25～102.5μg/ml(r=0.9997),大黄素在10.89～108.9μg/ml(r=0.9996),大黄酚在14.95～149.5μg/ml(r=0.9999)线性良好;平均回收率分别为98.9%(RSD=0.67%)、99.3%(RSD=1.56%)、101.5%(RSD=2.53%)、100.9%(RSD=0.40%)。结论该方法简便、可靠、准确,可用于黄龙胶囊的质量控制。

Objective To simultaneously establish a HPLC method for the determination of Aloeemodin, Rhein,Emodin and Chrysophanol in the Huanglong capsule. Methods The determination was performed on Aglient XDB C18 column （250 mm× 4.6 mm,5 μm）with mobile phase consisted of methanol -0.05% phosphoric acid （86：14 ）at a flow rate of 1.0 ml/min. The UV detection wavelength was set at 254 nm and column temperature： 35 ℃. Results The calibration curves were linear in the range from 16.28 - 162.8 μg/ml （r = 0. 9994 ）for Aloeemodin, from 10.25 - 102.5 μg/ml （r = 0. 9997 ） for Rhein, from 10.89 - 108.9μg/ml （ r = 0. 9996 ） for Emodin and from 14.95 - 149.5μg/ml （ r = 0. 9999 ） for Chrysophanol. The average recovery was 98.9% （ RSD = 0.67% ） ,99.3% （ RSD = 1.56% ）, 101.5% （ RSD = 2.53% ）, 100.9% （ RSD = 0.40% ）. Conclusion The determination method is simple, reliable, accurate, and it can be used for qual-ity control of Huanglong capsule.