卡托普利对腹主动脉缩窄大鼠模型心肌Ⅰ和Ⅲ型胶原及其mRNA表达的影响

Effect of captopril on myocardial collagen type Ⅰ and type Ⅲ and their mRNA expressions in rat with abdominal aortic banding

目的:探讨卡托普利对腹主动脉缩窄大鼠模型心肌Ⅰ和Ⅲ型胶原的改变及其mRNA表达改变的干预作用。方法:模型组根据Doering等方法,用银夹造成腹主动脉部分狭窄(银夹内径0.7 mm)。8周后免疫组织化学法进行Ⅰ型和Ⅲ型胶原染色,并用光密度值作为其含量指标行图像分析,即时PCR扩增后分析并测定胶原mRNA。结果:模型组造模8周时Ⅰ型和Ⅲ型胶原平均光密度及其mRNA表达较假手术组显著升高(P<0.01)。胶原组织化学染色示模型组较假手术组Ⅰ型和Ⅲ型胶原明显增多、增粗及排列紊乱;卡托普利组较模型组胶原明显减少。结论:大鼠腹主动脉缩窄导致反应性纤维化心肌中Ⅰ型和Ⅲ型胶原及其mRNA表达升高;卡托普利延缓甚至逆转心肌纤维化进程。

Objective：To investigate the effect of captopril on collagen type Ⅰ and type Ⅲ and their mRNA expressions in abdominal aortic banding model rats.Methods：Myocardial fibrosis rat model was established by partially banding abdominal aorta with silver clip（inside diameter 0.7 mm）according to Doering′s method.Optical density values of collagen typeⅠand type Ⅲ were measured by immunohistochemistry staining after 8 weeks.The mRNA expressions of collagen typeⅠand type Ⅲ were analyzed by real-time PCR amplification.Results：In the 8th week,compared with sham operation group,the average optical density value and mRNA expressions of collagen type Ⅰ and type Ⅲ were increased obviously in model group（P0.01）.The immunohistochemistry staining displayed that collagen type Ⅰand type Ⅲ were deposited,thicken and confused in myocardium stroma;and the quantity of collagen was decreased obviously in model group.Conclusions：The quantity of collagen type Ⅰ and type Ⅲ and the related mRNA expressions were increased in myocardical fibrosis induced by abdominal aortic banding in rats;captopril could delay even reverse the process of myocardial fibrosis.