摘要
根据微生态理论,文章选用嗜酸乳杆菌、纳豆芽孢杆菌、酿酒酵母作为制备复合微生物饲料添加剂的试验菌株。经一系列实验,结果表明:所选3种试验菌均能耐受0.3%的猪胆盐,对pH值为3.0的酸性环境也有较强的抵抗力,活菌数均在106CFU/ml以上;3种试验菌对人工胃肠液均有理想的耐受能力,能顺利通过胃、十二指肠到达小肠发挥作用,并能抑制猪体常见致病菌,是制备复合微生物饲料添加剂的优良菌种。
According micro-ecological theory,Lactobacillus delbrueckii,Saccharomyces cerevisiae and Bacillus natto were used as strains of microbial forage additive. The results indicated that these three strains survived well both in 0.3% of pig bile salt and pH 3.0,and the living bacterial number could be above 106 CFU/ml. They have good tolerance to artificial gastric juice and artificial intestinal juice,and the survival rates of three strains were over 70% and 80% in two juices. Furthermore,their mixed zymotic fluid can inhibit the pathogenic bacteria of pig. So,these three kinds of bacteria are the fine strains of complex microbial feed additive.
出处
《饲料工业》
北大核心
2010年第20期37-41,共5页
Feed Industry
关键词
微生物饲料添加剂
MTT比色法
人工胃液
人工肠液
microbial forage additive
MTT colorimetry
artificial gastric juice
artificial intestinal juice
