蓝莓优质苗组培技术研究

Study on Tissue Culture of Quality Blueberry Plantlets

为探索蓝莓优质苗综合培育技术,对蓝莓组培苗进行了培育技术研究。结果表明：蓝莓组培幼苗在温度15℃左右、湿度80%左右、12-20 d可达到炼苗驯化目的,炼苗适宜透光度为30%左右;优质成品苗培育初期移植选用T72、T50穴盘容器,基质选用腐殖菌根土＋珍珠岩（3∶2）;培育P＋1苗型选用14 cm×14 cm塑料钵,基质选用腐殖菌根土＋黄心土（4∶1）,透光度以50%为宜,速生期用CO（NH2）23.42 g/m^2,Ca（H2PO4）24.7 g/m^2,K2SO41.43 g/m^2,微量元素肥（B、Mn、Zn、Cu）0.03 g/m^2配成液肥施用。

The results from studying the comprehensive culture technique of quality blueberry plantlets showed that the optimum condition for acclimating plantlets was 15℃,80% humidity and 30% transmittance for 12～20d,the plantlets were transplanted in T72 or T50 trays with 3 humic mycorrhiza soil ∶2 pearlite at preliminary culture stage,the plantlets were transplanted in a plastic pod（14cm×14cm）with 4 humic mycorrhiza soil∶1 yellow subsoil under 50% transmittance for culturing plantlets of P＋1 type,and the plantlets at fast growth stage should be applied by fertilizer solution with 3.42g/m^2 CO（NH2）2,4.7 g/m^2 Ca（H2PO4）2,1.43g/m^2 K2SO4,0.03g/m^2 trace-element fertilizer.