β1整合素表达下调对人结肠癌细胞侵袭和耐药的影响

Effect of down-regulation of integrin-β1 expression on cell invasion and chemotherapy-resistance of human colon carcinoma HT-29 cell

目的 观察β1整合素表达下调对人结肠癌HT-29细胞侵袭和耐药的影响.方法 反义寡核苷酸技术转染HT-29细胞,逆转录-聚合酶链反应（RT-PCR）检测β1整合素表达下凋.Transwell侵袭窒方法检测HT-29体外侵袭力.流式细胞术（FCM）和噻唑蓝（MTT）比色法检测不同剂量的化疗药物[5-氟尿嘧啶（5-Fu）]对各组凋亡率和生长抑制率的影响.结果 实验组HT-29细胞βl整合素mRNA表达显著降低,体外侵袭实验迁移的HT-29细胞数（59±12）明显减少（P＜0.05）,在高浓度5-Fu（100、200 mg/L）时HT-29细胞的生长抑制率（36.97%和47.58%）和细胞凋亡率[（15.7l±1.46）%、（27.82±1.58）%]与对照组比较差异有统计学意义（P＜0.05）.结论 β1整合素表达下调可降低结肠癌细胞侵袭及化疗药物耐药性.

Objective To investigate the effects of down-regulation of integrin-β1 expression on cell invasion and chemotherapy-resistance of human colon carcinoma cell line HT-29. Methods The down-regulation of integrin-β1 mRNA expression by antisense oligonucleotide technologies was determined with reverse transcription-polymerase chain reaction （RT-PCR）. The invasive ability in vitro was evaluated by Transwell invasion chamber assay. Growth inhibition rates and apoptosis rates of HT-29 cells under different dosages of chemotherapeutic agents [5-fluorouracil （5-Fu）] were measured by methyl thiazol tetrazolium （MTT） colorimetric assay and flow cytometry （FCM）. Results In contrast with control group,the expression of integrin-β1 mRNA of HT-29 cells were markedly down-regulated,and the number （59 ±12） of migrated HT-29 cells was significantly decreased in the experimental group （P 〈 0. 05 ）. Higher dosages （ 100 mg/L and 200 mg/L of 5-Fu） caused a greater increase of inhibition in experimental group than in control （ P 〈 0. 05 ）. Conclusion The results demonstrate that down-regulation of integrin-β1 expression can effectively decrease cell invasion and chemotherapy-resistance of human colon carcinoma cell line HT-29.