乳及乳制品中L-羟脯氨酸的测定

Determination of L-hydroxyproline in milk and dairy products

采用比色法对乳及乳制品中的L-羟脯氨酸质量分数进行测定。结果表明,本方法的检出限为2.1μg/g,回收率为91.6%～101.3%,RSD为1.71%～2.45%,可用于判定乳及乳制品中是否添加动物胶原水解蛋白或者含有游离L-羟脯氨酸。

The L-hydroxyproline content in the milk and dairy products were determined by colorimetry mehod in this study.The result showed that the method had a good detection limit（2.1 μg/g）.The recovery ratios and RSD were 91.6%～101.3% and 1.71%～2.45%,respectively.This method can be used for identify whether the addition of animal protein hydrolysate or L-hydroxyproline in the milk and dairy products.