摘要
目的动态观测UC大鼠结肠黏膜IL-1βmRNA表达的变化及药物的影响。方法采用TNBS灌肠法复制UC大鼠模型,并采用逆转录-聚合酶链反应(RT-PCR)的方法半定量检测结肠黏膜IL-1βmRNA的表达。结果与正常组比较,模型大鼠结肠黏膜IL-1βmRNA表达在3,7,14 d续增高(P<0.01);与模型组比较,溃结灵或SASP在3,14 d可显著降低结肠黏膜致炎因子IL-1βmRNA的表达(P<0.05,P<0.01),而在7 d时作用不明显。结论致炎因子IL-1β在TNBS急性UC模型中持续参与炎症及结肠黏膜的损伤过程;在炎症发生发展的过程中,早期是药物干预的关键时期,而恢复期同样需要持续用药。
Objective to survey the expression of interleukin 1β（IL-1β）mRNA in colonic mucosa of ulcerative colitis（UC）rats and to observe the influence of Chinese medicine Kuijieling.Methods Rats UC model was established by rectal enema of trinitro-benzene-sulfonic acid.The expression of IL-1β in rats colonic mucosa was detected by semi-quantitative RT-PCR.Results Compared with the normal group,IL-1β expression in colonic mucosa of model rats was increased continuously on days 3,7,14（P〈0.01）.Compared with the model group,IL-1β expression in Kuijieling group and salicylazosulfapyridine group decreased significantly after treatment with Kuijieling decoction or SASP for 3 and 14 days（P〈0.05 or P〈0.01）,and the effect on IL-1β expression was not obvious on day 7.Conclusion Inflammatory factor IL-1β plays an important role in the progress of colonic inflammation and mucosa lesion of UC rats.Early intervention with medicine can counteract the progress of inflammation,and continuous medication still is necessary during the convalescence stage.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第6期592-594,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(30772754)
