摘要
以北京市怀柔区野生板栗的根部为试材,通过分离、纯化和培养获得培养性状与已知菌不同的3株菌种:菌A、菌B和菌C。采用双因素随机区组设计,将菌A、菌B、菌C和已知与板栗形成良好共生关系的红绒盖牛肝菌(X.c)制成菌液和固体菌剂,对板栗幼苗进行接种试验,之后测定苗木的生长指标,研究菌根的回接效应。结果表明:所有菌种固体菌剂的侵染率均高于液体菌剂;菌A的侵染率明显高于菌B、菌C和菌X.c,达到90.75%,分别提高11.1%、39.2%和12.75%。同时,菌A显著促进苗木地径生长,生物量最大,是值得应用推广的菌种。
This paper used the roots of wild chestnut in Huairou district as materials,through separation,purification,and cultivation getting three new mycorrhiza fungi:A,B and C.To adopt Two-factor randomized block design,Making inoculant by mycorrhiza fungi A,B,C and Hong Rong cap boletus(Xc),inoculated mycorrhizal fungi on chestnut seedlings,then mensurating nursery stock growth index,researching the effect of mycorrhiza-back.The results showed that all mycorrhiza fungi,their solid inoculant infection rates were higher than the liquid inoculant;infection rate of mycorrhiza fungi A was significantly higher than mycorrhiza fungi B,C and Xc,up to 90.75%,increasing respectively 12.75%,39.2% and 11.1%.Meanwhile,mycorrhiza fungi A significantly promoted seedling diameter growth,biomass maximum.Mycorrhiza fungi A was worth application and extension,but needing further identification.
出处
《北方园艺》
CAS
北大核心
2010年第21期18-21,共4页
Northern Horticulture
基金
"十一五"国家科技支撑计划资助项目(2006BAD18B0202)
关键词
板栗
菌根菌
侵染率
回接效应
Castanea mollissima
mycorrhiza
infection rate
effect of mycorrhiza-back