摘要
为探讨番茄类囊体膜脂ω-3脂肪酸去饱和酶基因（LeFAD7基因）在干旱胁迫下对番茄抗旱性的影响,以野生型（WT）和转正义LeFAD7基因株系T（＋）-12为试材,测定了番茄叶片类囊体膜脂脂肪酸组成,干旱胁迫后的光合参数、叶绿素荧光参数和活性氧清除酶（SOD、CAT和POD）活性的含量变化。结果表明：转正义基因番茄植株类囊体膜脂中亚麻酸（18∶3）含量升高,亚油酸（18∶2）含量下降,导致膜脂脂肪酸不饱和度升高。与WT相比,干旱胁迫下转正义基因番茄植株的PSⅡ最大光化学效率（Fv/Fm）和光合速率（Pn）下降程度较小,转正义基因番茄植株能维持较高的抗氧化酶活性。干旱胁迫下LeFAD7基因对番茄起一定的保护作用。
In order to investigate effact of LeFAD7 gene of thylakoid membrane on droug resistanc of tomato plants,WT and sense-transgenic lines T（＋）-12 of tomato were used to measure the fatty acid composition of thylakoid membrane,photosynthetic and Antioxidant enzyme activIty under drought stress.The results showed that linolenic acid（18∶3） obviously increased and the content of oleic acid（18∶2）concomitantly reduced in the sense-transgenic lines,resulting in the increase of the unsaturation of thylakoid memberrane lipids.The maximal photochemical efficiency of PS Ⅱ（Fv/Fm） and photosynthetic rates（Pn） in T（＋）-12 lines with higher 18∶3 decreased less under drought stress.under drought stress.Compared to WT,the sense-transgenic lines could maintain higher activities of the vigour of antioxidant enzyme.Those results indicated that LeFAD7 gene of thylakoid membrane lipids protect tomato plants on drought stress.
出处
《北方园艺》
CAS
北大核心
2010年第21期145-149,共5页
Northern Horticulture
基金
国家自然科学基金资助项目（30471053）
关键词
LeFAD7基因
类囊体膜脂组成
荧光参数
光合速率
抗氧化酶
干旱胁迫
LeFAD7 gene
composition of fatty acid in thylakoid membrane
fluorescence parameter
antioxidant enzyme
drought stress
