UV-B增强对南极小球藻的光合活性和抗氧化酶的影响

Effects of UV-B Stress on Photosynthetic Activity and Antioxidase of Chlorella vulgaris Antarctic Strain

以经过UV-B适应性锻炼的南极小球藻为研究对象,以同样经过UV-B适应性锻炼的普通小球藻为对照,用不同剂量的UV-B进行辐射处理,分别对南极小球藻和普通小球藻的细胞色素吸收光谱、光合放氧速率、电子传递链和抗氧化酶进行了比较研究,结果表明:在增强的UV-B辐射下,南极小球藻细胞色素、光合放氧机构受到的损害小于普通小球藻的,而抗氧化酶活性显著高于普通小球藻,抗氧化酶起到了一定的修复UV-B损伤的作用,南极小球藻具有一定的抗UV-B辐射能力.

The experiments of UV-B on Chlorella vulgaris Antarctic strain and Chlorella vulgaris have been carried on which both had physical training to UV-B.The results showed that：Absorption spectrum of pigment declined during 24 h UV-B exposure.The declining range of Chlorella vulgaris Antarctic strain was smaller than Chlorella vulgaris.The photosynthetic oxygen enolution rate of Chlorella vulgaris Antarctic strain and Chlorella vulgaris were inhibited by 54.27% and 74.8% respectively after 80 min UV-B treatment.The activities of MV-H2O,PSⅠ,PSⅡ declined under 80 μW·cm-2 light intensity of UV-B for 30 min UV-B treatment,but the activities of MV-H2O,PSⅠ,PSⅡ of Chlorella vulgaris dramatically declined were.Both CAT（catalase） activity and SOD（superoxide dimutase） activity increased at first,then decreased while the activities of Chlorella vulgaris Antarctic strain increased dramatically.Conclusion：Both Chlorella vulgaris Antarctic strain and Chlorella vulgaris were damaged by UV-B but the damage on Chlorella vulgaris Antarctic strain was smaller.Anti-oxidase played a protected role.Compared to Chlorella vulgaris,Chlorella vulgaris Antarctic strain to bear UV-B was stronger.