RASSF1A、CK19和p53在甲状腺良恶性病变中的表达及临床意义

Expression and Significance of RASSF1A、CK19 and P53 in Benign and Malignant Thyroid Tissues

目的探讨Ras相关区域家族1A(RASSF1A),细胞角蛋白19和p53基因产物在甲状腺乳头状癌及良性病变中的表达及临床意义。方法采用半定量逆转录聚合酶链反应(RT-PCR)检测,41例甲状腺乳头状癌、19例结节性甲状腺肿、35例甲状腺腺瘤和5例桥本氏病患者标本中RASSF1A、细胞角蛋白19和p53基因的表达情况。结果 RASSF1A、CK19和p53阳性表达率甲状腺乳头状癌与滤泡源性良性病变比较差异有显著性(P<0.05);而各良性病变中其表达率差异无显著性(P>0.05)。半定量结果显示,在甲状腺乳头状癌及良性病变中,上述3种基因产物的mRNA丰度差异性显著(P<0.01)。在甲状腺滤泡源性良恶性病变中RASSF1A与CK19表达呈负相关(γ=-0.3181,P=0.0013);RASSF1A与p53表达呈正相关(γ=0.2364,P=0.0179)。结论 RASSF1A、CK19和p53表达产物可作为鉴别甲状腺乳头状癌与良性乳头状增生的重要参考标记物,联合测定效果更好。

Objective To detect the expression of RASSF1A,CK19 and p53 genes＇products in papillary thyroid carcinoma and some other thyroid benign lesions and discussing the value of clinical diagnosis.Methods The expression of RASSF1A,CK19 and p53 was detected by semi-quantitative reverse transcriptase PCR in 23 case of patients with papillary thyroid cancer,16 cases of nodular goiter,29 cases of thyroid adenoma and 5 cases of patients with Hashimoto＇s disease.Results Expression of RASSF1A,CK19 and p53 were obviously different between papillary thyroid carcinoma and follicular benign lesions（P 0.05）.There was no difference in each variety of those benign lesions（P 0.05）.And semi-quantitative result showed that the abundance of mRNA of the three kinds of gene were significant different in papillary thyroid carcinoma and follicular benign lesions（P 0.01）.In thyroid benign and malignant lesions,the expression of RASSF1A and CK19 was negatively correlated（γ =-0.3181,P = 0.013）,RASSF1A and p53 expression was positively correlated（γ = 0.2364,P = 0.0179）.Conclusion RASSF1A,CK19 and p53 can be acted as useful markers in differential diagnosis of papillary thyroid carcinoma,and combined test can take more reliable results.