头孢类抗生素对中黑防杨叶片分化的影响

Effect on differentiation of Populus deltoides×P. cathayana leaves caused by cephalosporins antibiotics

探讨了头孢唑啉钠、头孢曲松钠、头孢霉素3种头孢类抗生素对农杆菌EHA105的抑制作用,以及对中黑防杨叶片生长和分化的影响。结果表明:头孢霉素和头孢曲松钠的抑菌效果优于头孢唑啉钠,但二者对叶片分化的抑制性太强,而750 mg/L的头孢唑啉钠对叶片分化影响很弱,仅比对照低3%,同时抑菌效果好。因此确定了由农杆菌介导的中黑防2号杨遗传转化时的抗生素为头孢唑啉钠其使用浓度为750 mg/L。

This paper discusses the cefazolin sodium,ceftriaxone sodium,and cefotaxime,three kinds of cephalosporin antibiotics on the inhibition of agrobacterium EHA105,and effect on the growth and differentiation of Populus deltiodes × cathayana leaves.The results showed that： cefotaxime and ceftriaxone sodium are superior to the inhibitory effect of cefazolin sodium,but the leaves differentiation inhibition caused by the two pairs of cephalosporin antibiotics is too strong,while 750 mg/L of cefazolin sodium has very weak influence on the differentiation of leaves,only 3% lower than that of comparison,and inhibitory effect is satisfactory.Thus it is determined that the genetic transformation antibiotics of agrobacterium-mediated Populus deltiodes×cathayana is cefazolin sodium,whose concentration for use is 750 mg/L.