运动发酵单胞菌表达载体的构建

Construction of expression vectors for Zymomonas mobilis

能源和环境问题是目前制约世界经济发展的两大难题,利用微生物生产燃料乙醇,已引起各国的普遍关注。运动发酵单胞菌(Zymomonas mobilis)是目前发现的乙醇发酵能力最强的微生物之一,但其只能以葡萄糖、果糖和蔗糖为底物。为了扩大运动发酵单胞菌的底物利用范围,本研究构建了重组表达载体pBPSG、pBPSE、pBPSGE,将瑞氏木霉内切葡聚糖酶基因和黑曲霉葡萄糖淀粉酶基因引入运动发酵单胞菌ATCC31821,在运动发酵单胞菌内源强启动子和转录终止信号的调控下获得表达,在胞外和胞内都检测到了活性,这为下一步试验打下基础。

In the recent years,fermentative production of ethanol from renewable resources had received attention due to increasing petroleum shortage.For the last two decades,ethanol production by the bacterium Zymomonas mobilis had been studied extensively.Wild-type Zymomonas mobilis could utilize only three substrates（sucrose,glucose,and fructose） as sole carbon sources,which were largely converted into ethanol and carbon dioxide.In order to broaden the substrates utilization range of this organism,genetic manipulation of these strains was carried out.The paper constructed expression vector contained the strong native promotor for the pyruvate decarboxylase and the foreign gene а-amylase and amyloglucosidase.