积雪草苷对小鼠脓毒症致急性肝损伤的保护作用研究

The Protective Effect of Asiaticoside on Sepsis-induced Acute Liver Injury in Mice

目的研究积雪草苷对小鼠脓毒症致急性肝损伤的保护作用,并初步探讨其作用机制。方法建立盲肠结扎穿孔(CLP)诱导的小鼠脓毒症致急性肝损伤模型,随后用不同剂量的积雪草苷(15,45 mg/kg)干预,24 h后检测血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、肿瘤坏死因子-alpha(tumor necrosis factor alpha,TNF-α)的含量;免疫印迹法检测肝组织中环氧合酶-2(cyclooxygenase-2,COX-2)的表达,并取部分肝组织进行组织病理学分析。结果积雪草苷剂量依赖性地减少脓毒症诱导的血清中ALT、AST及TNF-α的升高,抑制肝组织中COX-2蛋白的表达,病理切片显示积雪草苷组肝组织受损程度较模型组为轻。结论积雪草苷对CLP诱导的肝损伤具有一定的保护作用,其机制可能与抑制炎症介质COX-2和TNF-α有关。

Objective To study the protective effect and mechanism of asiaticoside on sepsis-induced acute liver injury in mice.Methods Sepsis-induced acute liver injury was induced by cecal ligation and puncture（CLP） in Kun Ming mice.Subsequently,different doses of asiaticoside（15 mg/kg,45 mg/kg） were used to intervene.24h later,serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and tumor necrosis factor alpha（TNF-α）levels were measured,western blotting was used to determine cyclooxygenase-2（COX-2） expression in liver tissues and histopathology examination was performed by hematoxylin-eosin（HE） staning method.Results The results showed that asiaticoside decreased serum ALT,AST and TNF-α levels,inhibited COX-2 expression in liver tissues,and histopathological changes of liver were improved by asiaticoside as compared to model group,and these effects were dose-dependent.Conclusion Asiaticoside has protective effect against sepsis-induced acute liver injury,and the mechanism is probably associated with the inhibition of TNF-α in serum and COX-2 expression in liver tissues.