c-Myc c-Fos c-Jun c-H-Ras在糖尿病早期大鼠肾小管和集合管上皮细胞内表达

Expression of c-Myc,c-Fos,c-Jun and c-H-Ras in the epithelial cells of rat renal and collecting tubulesin the early phase of diabetes mellitus

为了解ｃ－Ｍｙｃ、ｃ－Ｆｏｓ、ｃ－Ｊｕｎ、ｃ－Ｈ－Ｒａｓ是否参与７～１４０ｄ糠尿病早期大鼠肾小管、集合管上皮细胞两次转换率加速过程，我们设计了本实验。雄性Ｗｉｓｔａｒ大鼠６０只，四氧嘧啶（ａｌｏｘａｎ）尾静脉注射（５０ｍｇ／ｋｇ）造糖尿病模型；石蜡包埋肾切片经ｃ－Ｍｙｃ、ｃ－Ｆｏｓ、ｃ－Ｊｕｎ、ｃ－Ｈ－Ｒａｓ免疫组化染色。在７～３０ｄ、７０～１４０ｄｃ－Ｍｙｃ、ｃ－Ｆｏｓ、ｃ－Ｊｕｎ、ｃ－Ｈ－Ｒａｓ分别在近端小管、髓襻、远端小管、集合管上皮细胞内表达。ｃ－Ｍｙｃ、ｃ－Ｆｏｓ、ｃ－Ｊｕｎ在糖尿病早期肾小管、集合管上皮细胞内表达呈现延迟性、持续性、区域性和时间性。ｃ－Ｍｙｃ、ｃ－Ｆｏｓ、ｃ－Ｊｕｎ、ｃ－Ｈ－Ｒａｓ可能参与调控糖尿病早期肾小管。

OBJECTIVE To show whether or not c-Myc,c-Fos,c-Jun and c-H-Ras play a role in the turnover acceleration of renal and collecting tubular epithelial cells in the diabetic (7～140days)rat.MATERIALS AND METHODS 60 male Wistar rats were injected with alloxan(50mg/kg)via the tail vein;the paraffin-embedded sections of kidneys were observed with c-Myc,c-Fos,c-Jun and c-H-Ras immunohistochemical staining.RESULTS:c-Myc,c-Fos,c-Jun and c-H-Ras were expressed in the epithelial cells of the proximal tubules,the medullary loops,the distal and collecting tubules in 7～30 and 70～140 days,respectively.CONCLUSION:Expression of c-Myc,c-Fos,c-Jun and c-H-Ras displayed delayed,sustained,regional and timed properties.c-Myc,c-Fos,c-Jun and c-H-Ras may participate in the process of turnover acceleration of the epithelial cells.