通痹灵对佐剂性关节炎大鼠滑膜细胞TNF-αmRNA表达的影响

Effects of Tongbiling on TNF α mRNA Expression in Synoviocytes from Adjuvant Arthritis Rat

类风湿性关节炎（ｒｈｅｕｍａｔｏｉｄａｒｔｈｒｉｔｉｓ，ＲＡ）是一种免疫介导的炎症性疾病，多种细胞因子如：ＩＬ－１、ＩＬ－６及ＴＮＦ单独或联合介导ＲＡ炎症组织损伤。其中ＴＮＦ在ＲＡ炎症、软骨吸收和骨破坏中起重要作用，是ＲＡ发病机理中居中心地位的促炎症...

The expression levels of TNF α mRNA in synoviocytes from adjuvant arthritis rats (AA) were measured by semiquantitative RT PCR.The results showed that the production of TNF α mRNA by synoviocytes from AA rats were greatly enhanced as compared with that of normal controls ( P <0.01).After treatment with Tongbiling (TBL),the expression levels of TNF α mRNA in synoviocytes were significantly decreased ( P <0.01).The effects could be observed in methotrexate treated grop ( P <0.05),but less than in TBL treated grop.Whereas indomethacin further enhanced the expression levels of TNF α mRNA in synoviocytes ( P <0.05).Our data suggest that abnormal expression of TNF α mRNA in synoviocytes plays an important role in the pathogenesis of AA rats.The mechanisms of the therapeutic effect of TBL on AA might be due to inhibit the expression levels of TNF α mRNA in synoviocytes,and then to decrease the production of TNF α.