矮牵牛茎尖诱导分化研究

Study on Induced Differentiation of Petunia hybrida Shoot Tip

[目的]推动矮牵牛组培的产业化,达到使其苗木生产快速、经济的目的。[方法]共配制5种MS培养基,选择无污染、生长健壮的矮牵牛茎尖作为外植体,进行诱导分化单因素试验,研究不同浓度的NAA对矮牵牛外植体诱导成活率的影响,进而选择适合矮牵牛诱导成活的最佳培养基。[结果]培养基中NAA浓度的差异对外植体成活率的影响较大。NAA浓度为0.5mg/L的培养基为最佳诱导成活培养基,NAA浓度为0.7及0.3mg/L的培养基对矮牵牛的作用仅次于NAA浓度为0.5mg/L的培养基,但NAA浓度为0.1及0.9mg/L的培养基处理效果不是十分理想。筛选出矮牵牛组培最佳诱导分化培养基为MS+NAA0.5mg/L+6-BA0.3mg/L+琼脂8g/L+蔗糖30g/L。[结论]研究结果可为矮牵牛的组织培养提供参考。

[Objective] The rapid and economic production of Petunia hybrida sapling would be realized through the research on the industrialization of its tissue culture.[Method] The introduction and regeneration of the Petunia hybrida stem-tip culturing in 5 media were experimented and the effect of the concentration of NAA on the survival rate of Petunia hybrida plantlet was researched.[Results] The concentration of NAA greatly affected the survival rate of the explants and the best concentration of NAA in introduction medium was 0.5 mg/L,then 0.7 mg/L or 0.3 mg/L,and the worst,0.1 mg/L or 0.9 mg/L.The best suitable regeneration medium was MS＋NAA 0.5 mg/L＋6-BA 0.3 mg/L＋agar 8 g/L＋sucrose 30 g/L.[Conclusion] The result could be referred for the Petunia hybrida tissue culture.