P^(16)蛋白在肝癌中的表达

Expression of P16 Protein in Hepatocellular Carcinoma

目的：探讨P16蛋白与肝细胞性肝癌生物学行为的关系。方法：应用P16多克隆抗体对60例肝癌用S－P法进行免疫组织化学标记。结果：60例肝细胞性肝癌中有39例P16蛋白阳性（65％）。根据肿瘤大小分为3组，即肿瘤直径＜3cm，直径3～5cm及直径＞5cm。其P16表达阳率率分虽为75％、62.5％、60％，三者之间无显著性意义（P＞0．05）。根据组织学类型分梁状型、假腺管型、实团型与纤维硬化型，其P16表达阳性率分别为95.2％、81．8％、36.4％和33.3％。前两组与后两组之间有统计学差异（P＜0.01）。根据组织的分化程度，高分化（包括Ⅰ～Ⅱ级）P16阳性率明显高于低分化（包括Ⅲ～Ⅳ级）。结论：提示P16蛋白的表达与肝细胞性肝癌的分化程度有关，与肿瘤大小有关。

Purpose: To studay correlation of biological behavior of hepatocellular carcinoma(HCC) withexpression of P16 protein. Method: By S - P immunohistochemistry method,60 cases were marked by P16 multiclonal antibody. Results: The P16 protein were positive in 39 cases(the positive rate was 65 % ). according to thediameter of tumors,all cases were divided into three group: < 3cm. 35cm. and > 5cm. The opitive rates ofP16 were 75 %. 62. 5 % and 60%,there were no significant differrence(p >0. 05). according to the histologicalfeatures, the tumors were divided into trabecular. pseudo - glandular. compact and scirrhous types. The positiverates of P16 were 95. 2 %. 81. 8%. 36. 4% and 33. 3%. Comparing grade Ⅰ Ⅱ with grade Ⅲ Ⅳ,there wessignificant difference (p< 0. 01 ). Conclusion: The expression of P16 protein is related to the degree of the differentiation but not related to the size of the humors.