鸡新城疫的诊断及病理分析

Diagnosis and Pathological Analysis of Newcastle Disease

[目的]对疑似鸡新城疫病例进行诊断,从而对防控该病提供依据。[方法]应用常规病理剖检技术解剖死亡鸡只,观察病理变化。病死鸡组织脏器研磨等处理后接种10日龄SPF鸡胚,取尿囊液进行平板血凝试验(HA)与平板凝集抑制试验(HI)。取病鸡肠道、腺胃、脑、十二指肠、肺脏等组织制作石蜡切片,HE染色,镜检。[结果]HA和HI结果显示,收集的尿囊液可凝集鸡红细胞,同时这种凝集作用又可被抗新城疫阳性血清抑制。病理剖检可见,各组织脏器有明显的出血性病变,实质细胞均出现不同程度的损伤。[结论]该分离毒株被确诊为鸡新城疫病毒。

[ Objective ] To diagnose Newcastle disease and provide basis for its prevention. [ Method ] Dead chickens were dissected, and conventional pathological examination and pathological observation were performed. After the homogenate of dead chicken organs was inoculated in 10-day-old SPF chicken embryos, plate hemagglutination （HA） and plate agglutination inhibition test （HI） was carried out with allantoic fluid. The tissue of sick chicken, such as stomach, brain, duodenum, lung and other organizations, were collected and embedded in paraffin wax, H. E. staining and histopathological observation were finally performed. [ Result] The HA and HI restllts showed that chicken red blood cells could be agglutinated by allantoic fluid and this aggregation could be inhibited by positive sera against Newcastle disease. Additonally, all organs was present obvious hemorrhagic lesions and the parenchyma cells were damaged at different degrees. [ Conclusion ] The isolated virus in the test is identified as Newcastle disease virus.