弓形虫急性感染对小鼠血清和睾丸氧自由基水平的影响

Acute Infection of Toxoplasma gondii Affects the Level of Oxygen Free Radicals in Serum and Testes of Mice

目的观察弓形虫感染对小鼠血清及睾丸的氧自由基和抗氧化酶水平的影响。方法将40只雄性BALB/c小鼠随机均分为4组,每组随机抽取5只腹腔注射弓形虫速殖子(2.5×103个/只,溶于0.2mlPBS),余5只为阴性对照,注射等量PBS。将各组小鼠分别于腹腔注射后第1、3、5和7天处死,取血和睾丸组织,检测血清和睾丸组织中一氧化氮(NO)、羟自由基(·OH)、抗超氧阴离子(O2-)自由基和超氧化物歧化酶(SOD)的含量。结果弓形虫感染组血清和睾丸组织中NO、·OH和O2-的浓度随时间延长逐渐升高,感染后第3、5和7天的浓度均显著高于对照组(P<0.01)。血清和睾丸组织内SOD浓度在感染后逐渐上升,第3天达峰值,后逐渐下降,感染后第3、5、7天与对照组差异有统计学意义(P<0.01)。结论弓形虫急性感染可引起小鼠血清及睾丸氧自由基(NO、·OH、O2-)和超氧化物歧化酶SOD水平的升高。

Objective To observe the impact of Toxoplasma gondii infection on the level of oxygen free radicals and antioxidant enzyme in serum and testes of mice. Methods 40 BALB/c male mice were randomly divided into four groups. Five mice from each group were injected intraperitoneally with 2.5×103 tachyzoites of T. gondii, the others received PBS. Mice were sacrificed on the 1st, 3rd, 5th, and 7th day after inoculation. Samples of serum and testes were collected to determine the content of oxygen free radicals and superoxide dismutase （SOD）. Results The concentration of the oxygen free radicals （NO, ·OH, O2-） in serum and testes of the mice increased along with the days of infection. The concentration of SOD reached a peak on the 3rd day after the injection and then decreased. Both of oxygen free radicals and SOD showed a statistical difference with the control （P0.01）. Conclusion Acute infection of T. gondii leads to an increase of oxygen free radicals and SOD in the serum and testes of mice.