阴道毛滴虫低温保存及其影响因素

Cryopreservation of Trichomonas vaginalis and Its Influence Factors

观察不同浓度二甲亚砜(DMSO)和甘油、不同密度滴虫及不同冻存时间对阴道毛滴虫在-78℃低温保存的影响。阴道毛滴虫的最适冻存密度为(1～2)×106/ml。10%甘油和10%二甲亚砜的冻存效果最好,复苏存活率分别为38.0%和31.7%,两者差异无统计学意义(P>0.05)。短期冻存(1～16周)的效果良好,均可在37℃重悬培养至对数生长期。

Different concentrations of dimethylsulfoxide （DMSO） and glycerol, various densities of Trichomonas vaginalis trophozoites and duration of storage were used to study the effects on the cryopreservation of T. vaginalis at -78 ℃. The optimal densities of trichomonads for cryopreservation were （1-2） ×106/ml. Glycerol and DMSO showed a high protective effect, with a maximum effect at a concentration of 10% , and the survival rates were 38.0% and 31.7% , respectively. There was no significant difference between them（P0.05）. The results showed that the above optimized factors can provide a good protection in short-term （1-16 weeks） cryopreservation, and the trichomonads can be incubated to a logarithmic growth phase at 37 ℃.