分泌性中耳炎豚鼠模型鼓室黏膜上皮和血清中TGF-β的表达及意义

Expression and Significance of Transforming Growth Factor-β in the Tympanum Mucosa of Middle Ear and Venous Serum in Otitis Media with Effusion in the Guinea Pig Model

目的探讨TGF-β在分泌性中耳炎发生和转归中的作用。方法 100只豚鼠分为两组,A组(对照组)50只随机分为5组,每组10只,不作任何处理;B组(实验组)50只,以肺炎链球菌鼓室注射制造豚鼠分泌性中耳炎模型,再分为注入后1、3、7、14、30天组,每组10只。对照A组随同B组各组分别用ELISA和Westernblot检测其鼓室黏膜上皮及血清中TGF-β1、TGF-β2的含量。结果 B组豚鼠鼓室黏膜上皮及血清中TGF-β1表达在第1、3天与对照组无明显差异,第7天表达明显,之后逐渐增强,第30天达高峰,组间差异有统计学意义(P<0.001),7、14、30天组与对照组比较差异有统计学意义(P<0.5)。B组豚鼠鼓室黏膜上皮及血清中TGF-β2表达在第1天之后逐渐增强,第14天达高峰后开始降低,各时间组组间差异有统计学意义(P<0.001),与A组比较差异有统计学意义(P<0.05)。结论 TGF-β1、TGF-β2在豚鼠分泌性中耳炎模型不同时期鼓室黏膜上皮及血清中有不同表达,说明这两种因子在分泌性中耳炎的发生与转归中有着不同的重要作用。

Objective To explore the role of transforming growth factor β1（TGF-β1） and transforming growth factor-β2（TGF-β2） in the onset and development of otitis media with effusion （OME） in the guinea pig model.Methods The expression of transforming growth factor-β1 and transforming growth factor-β2 in the tympanum mucosa of middle ear and venous serum in guinea pigs with OME were examined by means of ELISA and Western blot.Results Compared with control group,expressions of TGF-β1 in the tympanum mucosa of middle ear and venous serum in test group were indistinctive at 1 d and 3 d,more intensive at 7 d,and reached maximum at 30 d.There was significant statistic difference among groups（P〈0.001）.Compared with control group,expressions of TGF-β2 in the tympanum mucosa of middle ear and venous serum in test group increased from 1 d,reached maximum at 14 d,then descended.There was significant difference among groups （P〈0.001）.Conclusion TGF-β1,TGF-β2 were expressed differently in the tympanum mucosa of middle ear and venous serum in the middle ear mucosa of the guinea pigs with OME at different stages.This indicates that TGF-β1,TGF-β2 may play a diverse and key role in the onset and development of OME.