可用于实时荧光定量PCR标准化的白桦内参基因

Selection of Internal Control Genes for Real-Time RT-PCR Normalization in White Birch(Betula platyphylla Suk.)

为了快速、准确的对白桦多个样品进行基因表达分析,本研究应用荧光定量PCR技术,对白桦持家基因微管蛋白基因(Tu)和泛素基因(UbQ)在不同处理的细胞及不同树龄或不同生长季节白桦植株各部位表达的稳定性进行检测。结果表明,对于茉莉酸甲酯(MeJA)、水杨酸(SA)处理的白桦细胞和不同生长季节白桦植株的基因定量表达分析,可同时使用Tu和UbQ平衡化qRT-PCR数据;而针对不同树龄白桦茎皮中基因表达研究时,单独使用UbQ基因作为内参对照即可获得精确的表达数据。

In order to facilitate rapid and accurate gene expression analysis of multiple samples from birch,the expression stability of housekeeping genes such as genes coding micro-tube protein（Tu） and ubiquitin（UbQ） were assessed by qRT-PCR using a set of cDNAs from different birch samples including cells after different treatments and different parts of birch plants in different ages or different growing seasons.The results suggested that the Tu and UbQ genes could provided superior transcript normalization in birch cells treated with different elicitors（MeJA and SA）,and birch plants in different growing seasons,while UbQ gene was the recommended reference gene for gene expression studies using cDNAs from white birch bark in defferent years.