三种不同因子对三角帆蚌外套膜细胞Ca^(2+)流动性的影响

The Effect of Three Different Factors on Extracellular Ca^(2+) Flux of Mantle Cell from Hyriopsis cumingii

三角帆蚌（Hyriopsis cumingii）是我国主要的育珠母蚌,其所培育出的珍珠色泽鲜艳、细腻光滑,因而成为目前淡水育珠生产中首选的育珠母蚌之一。蚌的外套膜是贝壳和珍珠形成的重要组织器官,有内外两层表皮细胞及其间的结缔组织构成。它对钙具有高度通透性,其上皮细胞具有通过细胞膜主动吸收Ca（2＋）和贮存Ca（2＋）的功能,并通过胞吐作用排出钙至外套膜外腔中,这些钙就是形成珍珠的基础。

In order to study the molecular mechanisms of calcium metabolism in mantle cell from Hyriopsis cumingii,Ca（2＋） flux direction and rate were detected by non-invasive micro-test technology（NMT）.Three mantle cell cultures were divided into three groups（3 reduplication/each group）.For the first group,the different concentrations of Ca^（2＋）（0.5 mmol/L,1.25 mmol/L,3 mmol/L） were added to culture medium;for the second group,the different concentrations of vitamin D_3（control,50U/L,100U/L,500 U/L） were added into culture medium;for the third group,cells and tissues were cultured in the different temperatures（20℃,26℃,30℃）,respectively.The results showed that extracellular Ca^（2＋） flux was gradual changed from efflux to influx,and movement rate increased when adding Ca^（2＋） into culture medium.The Ca^（2＋） flux of 3 mmol/L Ca^（2＋） adding group had significant difference compared with the control group（P0.01）.The movement of Ca（2＋） was gradual changed from efflux to influx,and movement rate increased when adding vitamin D_3 in cultured cells.Among of this,the Ca（2＋） flux of 500 U/L vitamin D_3 group had significant difference compared with the control group（P0.01）.Furthermore,culture temperature significantly affected the extracellular Ca^（2＋） flux of the mantle cells.The flow directions of extracellular Ca^（2＋）changed from efflux to influx with increasing temperature（P0.05）.The influx rate at 26℃was the biggest among all the groups（P0.05）.This experiment has a great significance on clarifing the formation and formation mechanism of pearls.