凋亡抑制基因Survivin和GW112在Barrett食管与食管腺癌中的表达及其意义

The expression and clinical significance of survivin and GW112 in Barrett's esophagus and esophageal adenocarcinoma

目的探讨凋亡抑制基因Survivin和GW112在反流性食管炎、Barrett食管及食管腺癌中的表达及其意义。方法采用SYBR Green I荧光定量RT-PCR方法检测反流性食管炎、Barrett食管、食管腺癌黏膜组织中SurvivinmRNA和GW112 mRNA的表达。结果 GW112在对照组和反流性食管炎阴性组中呈低水平表达,明显低于反流性食管炎阳性组、Barrett食管组及食管腺癌组的表达(P<0.05),反流性食管炎阳性组、Barrett食管组及食管腺癌组GW112表达水平无明显差异(P>0.05)。Survivin表达水平依正常对照、反流性食管炎阴性、反流性食管炎阳性、Barrett食管、食管腺癌的次序渐进增强,其中反流性食管炎阳性组、Barrett食管与食管腺癌组患者Survivin表达水平明显高于对照组和反流性食管炎阴性组(P<0.05,P<0.01),且3组间亦存在显著性差异(P<0.05,P<0.01)。结论 Survivin和GW112基因的过度表达引起的细胞凋亡抑制在Barrett食管及其腺癌发生、发展过程中起着重要的作用,尤其是Survivin在Barrett食管与食管腺癌的发生、发展最早步骤中可能扮演着更主要的角色。

Objective To explore the expression and significance of survivin and GW112 in development of reflux esophagitis,Barrett＇s esophagus and esophageal adenocarcinoma.Methods The expression levels of survivin and GW112 in mucosa tissues of patients with reflux esophagitis,Barrett＇s esophagus or esophageal adenocarcinoma were detected by real-time fluorescent quantitative PCR.Results The expression of GW112 mRNA in the Barrett＇s esophagus and esophageal adenocarcinoma tissues was significantly higher than that in the normal and reflux esophagitis-negative esophageal tissues（P0.05）,but it did not significantly differ between Barrett＇s esophagus and esophageal adenocarcinoma.The expression of survivin mRNA was significantly higher in Barrett＇s esophagus and esophageal adenocarcinoma tissues than in the normal and reflux esophagitis esophageal tissues（P0.05,P0.01）;moreover,it increased progressively with the development order of reflux esophagitis,Barrett＇s esophagus and esophageal adenocarcinoma,and was related to staging.Conclusion The overexpression of survivin and GW11 in Barrett＇s esophagus and esophageal adenocarcinoma suggests that survivin and GW112 gene may play an important role in the genesis and progression of Barrett＇s esophagus and esophageal adenocarcinoma.