茶多酚对人宫颈癌Hela细胞系生长的影响

The influence of tea polyphenols (TP) on human cervical cancer Hela cells growth

目的:探讨茶多酚(TP)对人宫颈癌Hela细胞生长的影响。方法:应用不同浓度的TP作用于人宫颈癌Hela细胞,采用四甲基偶氮唑蓝(MTT)法检测药物对细胞的增殖抑制率;流式细胞术(FCM)检测细胞凋亡、细胞周期分布。结果:TP能明显抑制Hela细胞的增殖,并呈剂量和时间依赖性;经IC50TP处理Hela细胞后,FCM分析发现实验组G0/G1期Hela细胞与对照组相比均有显著增多,G2/M期细胞逐渐减少;凋亡率明显高于对照组(P<0.05)。结论:TP能明显抑制人宫颈癌Hela细胞的增殖,诱导其凋亡。

Objective：To investigate the influence on human cervical cancer Hela cell growth induced by TP.Methods：Hela cells were treated with TP of the different concentrations.MTT method was used to detect the growth inhibition effects.Flow cytometry was used to detect the proliferation and apoptosis of the cells after staining by PI or Annexin V-FITC and PI.Results：The cell growth was inhibited significantly,with a time and dose dependent manner.After TP treatment,the apoptosis of Hela cells were induced.FCM analysis found that the number of treated cells in G0/G1 phase increased,but that in S and G2/M phase decreased.The apoptosis rate was significantly higher than that of control group（P0.05）.Conclusion：TP can significantly inhibit proliferation and induce apoptosis of Hela cells.