摘要
目的:研究急性白血病患者外周血淋巴细胞亚群、调节性T细胞水平的变化及临床意义。方法:采用流式细胞仪对60例急性白血病(AL)患者[急性髓细胞性白血病(AML)30例、急性淋巴细胞性白血病(ALL)30例]及40例正常人外周血淋巴细胞亚群及调节性T细胞水平进行检测。结果:初诊AL组与正常对照组比较,CD3+T淋巴细胞百分比、CD4+T淋巴细胞百分比、CD4+/CD8+比值及CD16+/56+NK细胞百分比均明显降低(P<0.05);CD8+T淋巴细胞百分比与CD19+B淋巴细胞百分比均明显升高(P均<0.01)。ALL组CD4+T淋巴细胞百分比及CD4+/CD8+比值明显低于AML组(P<0.01)。与对照组相比较,初诊AL患者外周血CD4+CD25+T细胞和CD4+CD25highTreg细胞均明显升高(P<0.01)。结论:AL患者细胞免疫功能明显异常;与AML患者相比,ALL患者细胞免疫功能更为低下。CD4+CD25highTreg细胞增多可能是AL患者免疫功能受抑的重要原因之一。淋巴细胞亚群及调节性T细胞水平检测在评价AL疗效及判断预后方面具有一定的临床价值。
Objective:To study the changes of lymphocyte subsets,regulatory T cells in peripheral blood of patients with acute leukemia(AL) and its clinical significance.Methods: The level of peripheral blood lymphocyte subsets,regulatory T cells of 60 AL patients and 40 normal controls were detected with flow cytometry.Results: Compared with the normal controls,the percentage of CD3+ T cell,CD4+Tcell,CD16+CD56+ NK cell and the ratio of CD4+/CD8+ decreased obviously in newly diagnosed AL group(P0.05),while the percentage of CD8+ T cell and CD19+ B cell increased significantly(P 0.01).The percentage of CD4+T cell and the ratio of CD4+/CD8+ in acute lymphoblastic leukemia(ALL) group were much lower than those in acute myelogenous leukemia(AML) group(P0.01).Compared with that of control group,the proportion of CD4+CD25 high Treg cells and CD4+ CD25+ T cells in newly diagnosed AL group were significantly increased(P0.01).Conclusion: Cellular immune function is significantly abnormal in patients with AL.Compared with AML,patients with ALL had more poor cellular immune function.The increased number of CD4+ CD25 high Treg cells might be one of the important reason of immunosuppression in AL.Detection of lymphocyte subsets and regulatory T cells are of clinical value on evaluation of therapeutic effect and prognosis of AL.
出处
《现代肿瘤医学》
CAS
2010年第11期2230-2233,共4页
Journal of Modern Oncology