逍遥散对产后抑郁大鼠海马中单胺类神经递质含量的影响

Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression

目的:研究逍遥散对产后抑郁大鼠海马中单胺类神经递质含量的影响。方法:将48只卵巢切除的雌性大鼠分为4组:对照组,模型组,氟西汀组(阳性对照组)和逍遥散组。前23 d,除对照组外,其余大鼠每天皮下注射雌激素模拟大鼠的怀孕状态;接下来的30 d,各组大鼠分别灌胃给予受试物。实验结束后,观察大鼠的行为学指标;取出海马组织,用酶联免疫法测定海马组织中单胺类神经递质去甲肾上腺素(noradrenaline,NE)、多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT)和5-羟吲哚乙酸(5-hydroxyindoleacetic acid,5-HIAA)的含量。结果:与对照组相比,模型组行为学表现明显处于产后抑郁状态,海马中5-HT、NE和DA显著降低(P<0.01),5-HIAA显著增加(P<0.01);而中药逍遥散可显著改善行为学指标,并增加海马组织中5-HT、NE和DA的含量,降低5-HIAA的含量(P<0.01)。逍遥散的效果与氟西汀类似。结论:逍遥散可显著调节大鼠海马中单胺类神经递质的含量,发挥抗抑郁作用。

Objective： To investigate the effects of Xiaoyao Powder,a compound traditional Chinese herbal medicine for treatment of depression,on monoamine neurotransmitters in hippocampus of rats with postpartum depression（PPD）.Methods： Ovariectomized Sprague-Dawley rats were randomly divided into 4 groups： control group,untreated group,fluoxetine hydrochloride group and Xiaoyao Powder group.Except for the control group,the rats in the other 3 groups were injected subcutaneously with estrogen for 23 successive days to induce the ＂pregnancy＂ state.In the next 30 successive days,rats were given test drugs respectively and the concentrations of noradrenaline（NE）,dopamine（DA）,5-hydroxytryptamine（5-HT） and 5-hydroxyindoleacetic acid（5-HIAA） in hippocampus were measured by enzyme-linked immunosorbent assay（ELISA）.Results： PPD was induced in rats of untreated group,and compared with rats of control group,the concentrations of NE,DA and 5-HT in the hippocampus significantly reduced（P0.01）,while the concentration of 5-HIAA significantly increased（P0.01）.Xiaoyao Powder treatment significantly improved the behavior indexes and levels of monoamine neurotransmitters in hippocampus（P0.01）.Xiaoyao Powder and fluoxetine hydrochloride produced similar effects in rats with PPD.Conclusion： Xiaoyao Powder produces an antidepression effect in rats by regulating the levels of monoamine neurotransmitters in hippocampus.