波动性高糖下血红素加氧酶-1对INS-1细胞凋亡相关蛋白的影响

Effects of heme oxygenase-1 on proteins related to apoptosis in INS-1 cells exposed to intermittent high glucose

目的探讨波动性高糖下血红素加氧酶-1(HO-1)对INS-1细胞凋亡相关蛋白的影响。方法实验以体外培养的INS-1细胞为研究对象,分为正常对照组、持续性高糖组、波动性高糖组、钴原卟啉(CoPP)+波动性高糖组、锌原卟啉(ZnPP)+波动性高糖组。各组均干预72h,Western blot检测HO-1蛋白表达,免疫细胞化学法检测Bax、Bcl-2蛋白的表达。结果与正常对照组相比,波动性高糖组、持续性高糖组HO-1表达显著增高,Bcl-2/Bax比值显著降低(P均<0.05)。与波动性高糖组相比,CoPP+波动性高糖组HO-1蛋白表达显著升高,Bcl-2/Bax比值亦明显升高(P均<0.05),而ZnPP+波动性高糖组则出现相反的变化趋势。结论波动性高糖可降低INS-1细胞凋亡相关蛋白Bcl-2/Bax比值,而HO-1可能通过上调Bcl-2/Bax比值对INS-1细胞发挥抗凋亡的保护效应。

Objective To study the effect of heme oxygenase-1（HO-1） on proteins related to apoptosis in INS-1 cells with exposure to intermittent high glucose.Methods INS-1 cells cultured in vitro were divided into control group,persistent high glucose group（PHG）,intermittent high glucose group（IHG）,CoPP ＋ intermittent high glucose group（CoPP＋IHG）,and ZnPP＋ intermittent high glucose group（ZnPP＋IHG）.After 72 h of treatment with the corresponding protocols,the cells were examined forexpressions of HO-1 protein by Western blotting and forexpressions of Bax and Bcl-2 by immunocytochemistry.Results In comparison with the control group,the cells in both PHG group and IHG group showed significantly increased expressions of HO-1（P0.01） and decreased Bcl-2/Bax ratios（P0.05）.The cells in CoPP＋ IHG group exhibited a greater HO-1 protein expression but a lowerBcl-2/Bax ratio than those in IHG group（P0.05） The ZnPP＋IHG group demonstrated opposite changes in terms of HO-1,Bax and Bcl-2 expressions compared with the CoPP＋IHG group.Conclusion Intermittent high glucose can lower Bcl-2/Bax ratio in INS-1 cells,and HO-1 may protect INS-1 cells against apoptosis possibly by up-regulating the Bcl-2/Bax ratio.