择时运动对昼夜节律基因表达的影响

Effect of chronoexercise on circadian expression of clock genes

目的研究择时运动调节昼夜节律的分子生物学机制。方法金黄地鼠在昼夜节律时相点CT6做力竭运动,2h后采用荧光定量RT-PCR技术,对(CT6)择时运动引起金黄地鼠自发性活动超前间脑中Per1mRNA(mPer1)、Per2mRNA(mPer2)表达水平进行测定。结果在CT6择时运动,金黄地鼠间脑中的mPer1、mPer2基因表达显著性降低。结论在CT6时相点力竭运动引起动物相位超前的分子生物学机制,可能是通过降低间脑细胞内昼夜节律基因转录-翻译负反馈调节环路实现的。

Objective To investigate the molecular biological mechanism of chronoexercise regulating circadian.Methods Expressions of mPer1 and mPer2 in the diencephalon of golden hamster were determined 2 hours after acute exhaustive exercise（circadian time 6） by quantitative RT-PCR.Results Chronoexercise at CT6 significantly decreased expressions of mPer1 and mPer2 in the diencephalon of golden hamster.Conclusion Inhibitory effect of chronoexercise on Per1 and Per2 mRNA levels in the diencephalon of golden hamster at CT6 may be achieved transcription-translation-based autoregulatory negative feedback loop.