雌激素对AD模型大鼠脑内P-Tau、ChAT和神经生长因子蛋白表达的影响、

Effects of estrogen on P-Tau,ChAT and nerve growth factor protein expressions in the brain tissue of rats with Alzheimer's disease

目的探讨雌激素治疗阿尔茨海默病(AD)动物模型后脑组织中磷酸化Tau(P-Tau)、乙酰胆碱转移酶(ChAT)和神经生长因子(NGF)蛋白的变化。方法大鼠右侧脑室注射Aβ1-42蛋白片段制作AD模型,2周后颈部皮下植入17β-雌二醇片剂30d。HE染色观察大鼠脑组织神经元的病理改变,免疫组化法观察P-Tau、ChAT和NGF蛋白的表达变化。结果 AD组大鼠脑组织出现神经纤维缠结,雌激素治疗组比AD组明显减少;治疗组磷酸化Tau蛋白表达比模型组明显减少(P<0.01),ChAT和NGF蛋白的表达比模型组明显增多(P<0.01)。结论雌激素能够上调NGF和ChAT蛋白的表达及抑制tau蛋白的异常磷酸化,对AD模型大鼠有明显的治疗作用。

Objective To examine the effect of estrogen on the expressions of phosphorylated Tau（P-Tau）,ChAT and nerve growth factor（NGF） protein in the brain tissue of rat models of Alzheimer disease（AD）.Methods Rat models of AD were established by injecting A？±-42 protein fragments in the right lateral ventricle.Two weeks later,17？-estradiol tablets were implanted subcutaneously at the neck of the rats and maintained for 30 days.The pathological changes in the rats＇ brain neurons and alterations in the expressions of P-Tau,ChAT and NGF proteins were observed using HE staining and immunohistochemistry,respectively.Results In the AD rats,neurofibrillary tangles occurred in the brain tissue,and estrogen treatment significantly reduced the formation of neurofibrillary tangles.Estrogen treatment also resulted in lowered P-Tau expression and increased ChAT and NGF protein expressions in comparison with those in the AD model rats.Conclusion Estrogen can up-regulate ChAT and NGF and down-regulate tau protein expression,thus producing obvious therapeutic