长途运输应激对两种品系五指山小型猪自主神经功能的影响

Effect of Long-term Transportation Stress on Autonomic Nervous Control of Heart in Two Strains of Wuzhishan Miniature Pigs

目的 观察长途运输应激对两种品系五指山小型猪自主神经功能的影响. 方法 基于无创遥测技术,监测清醒自由活动状态下经21 h长途运输应激后和2个月恢复后,两种品系五指山小型猪心电图（ECG）和活动的改变,并用HRV分析评价其自主神经功能状况. 结果 （1）小型猪对照组具有明显的昼夜节律变化,白天以交感神经活动为主,晚上以副交感神经活动为主,且Black品系RRI、RMSSD、STV、VLF、LF和LF/HF比值均显著低于white品系（P〈0.05,P〈0.01）,而HR、LTv、TP和HF均显著高于White品系（P〈0.05,P〈0.01）;（2）与对照组比,长途运输应激后.小型猪RRI、SDNN、RMSSD、STV、LTV、TP、vLF、LF和LF/HF比值均明显降低（P〈0.01）,HR和HF增加（P〈0.05,P〈0.01）,且Black品系LTS组RRI、RMSSD、STV、VLF、LF和LF/HF比值均显著低于white品系LTS组（P＜0.05,P〈0.01）,而HR、LIV、TP和HF均显著高于White品系LTS组（P〈0.05,P〈0.01）;（3）长途运输应激小型猪的活动、心率及其HRV时域和频域指标均具有显著的相关性（P〈0.05,P〈0.01）. 结论 长途运输应激能使不同品系五指山小型猪的自主神经活动产生明显的差异,且Black品系五指山小型猪更易受长途运输应激的损害;另外,交感神经活动的降低可能是导致运输应激致小型猪生物学功能发生改变的主要原因之一.

Objective To observe the effects of long-term transportation stress on autonomic nervous control of heart in two strains of Wuzhishan miniature pigs. Methods Electrocardiogram （ECG） and autonomic activities were recorded after long-term transportation stress for 21h and recovered, from conscious and unrestrained miniature pig by using a non-invasive telemetry system and the autonomic nervous function was investigated by power spectral analysis of heart rate variability （HRV）. Results （1） It demonstrates an obvious circadian rhythm in Wuzhishan miniature pig, and all of results suggest that the parasympathetic nervous activity is predominant during dark phase, and sympathetic nervous activity is predominant during light phase in miniature pigs. The RR interval （RRI）, the root-mean-square of successive differences （RMSSD）, short term variability （STV）, very low frequency （VLF）, low frequency （LF） and LF/HF in Black strain were significant lower than those of White strain （P〈0.05, P〈0.01）, while heart rate （HR）, long term variability （LTV）, total power （TP） and high frequency （HF） were significant higher than those of white strain（P〈0.05, P〈0.01）; （2） Compared with control group, RRI, SDNN, RMSSD, STV, LTV, TP, VLF, LF and LF/HF in wuzhishan miniature pig were significant decreased after long-term transportation stress （P〈0.05, P〈0.01）, while HR and HF were increased （P〈0.05, P〈0.01）.The Black strain of RRI, RMSSD, STV, VLF, LF and LF/HF in the long-term trans-portation stress group were significant lower than these of White strain （P〈0.05, P〈0.01）, while HR, LTV, TP and HF were significant higher than these of White strain （P〈0.05, P〈0.01）; （3） It has signifi- cant correlations between activity, heart rate and time and frequency domain indices （P〈0.05, P〈0.01）. Conclusion It has significant difference on autonomic nervous control of heart between two strains of wuzhishan miniature swine induced by long-term transportation stress, and Black strain maybe easy to damage the balance. Moreover, long-term transportation stress can induce the sympathetic nervous activity decreasing, it maybe a major reason to cause of changing the biological function of miniature pig induced by long-term transportation stress.