1.25（OH）2D3对NOD小鼠心肌超微结构的影响

Effect of 1,25（OH）2D3 on Myocardium in NOD Mice

目的 观察NOD小鼠在1,25（OH）2D3治疗前后心肌超微结构的改变,以探讨1.25（OH）2D3对心脏的保护作用. 方法 NOD小鼠（19只）用环磷酰胺诱发建立糖尿病模型后,随机分为1.25（OH）2D3治疗组及糖尿病模型组,每组6只.观察用药前及用药4周后糖尿病小鼠血糖及心肌超微结构的变化. 结果 模型组小鼠心肌纤维稀疏,肌丝扭曲部分断裂,z线增宽,M线、H带模糊不清.线粒体肿胀变形,嵴稀疏,排列紊乱,基底膜增厚,间质胶原纤维增生.1.25（OH）2D3治疗组小鼠心肌细胞中,肌丝排列整齐,结构清晰,肌纤维无明显断裂,线粒体肿胀较未治疗组明显减轻,心肌内微血管基底膜较模型组薄. 结论 1.25（OH）2D3可减轻实验性Ⅰ型糖尿病小鼠心肌超微结构的病变,对心肌有一定的保护作用.

Objective To study the heart protective effect of 1,25（OH）2D3 on diabetic NOD mice Methods A total of 19 NOD mice were enrolled in the study, all of them were treated to be the model of diabetes by injecting CTX, and then randomly divided into 1.25（OH）2D3 group and untreated group. Other 4 BALB/c mice were set as normal controls. Myocardial morphology was observed in all three groups after 4 weeks. Results The Electronic microscopic findings showed that after the treatment of four weeks with 1.25（OH）2D3, the heart pathological changes were markedly reduced as compared with those diabetic mice untreated by 1.25（OH）2D3. Conclusion Treatment with 1.25（OH）2D3 can reduce the injury of the heart and prevent the pathological progress of structure of heart in NOD mice.