羟乙基淀粉一步与两步法分离骨髓单个核细胞及血小板的比较

Mononuclear cells and platelets isolated from human bone marrow using hydroxyethyl starch by one-step way versus two-step way

背景:羟乙基淀粉和淋巴细胞分离液两步法分离骨髓有核细胞,可以提高单个核细胞的浓缩效率,但对血小板残留量的研究未见报道。目的:明确羟乙基淀粉两种分离方法浓缩骨髓单个核细胞的效率和残留血小板的量。方法:骨髓来自在新疆医科大学第一附属医院行成体自体骨髓有核细胞移植治疗相关疾病分离骨髓的检测标本。采用羟乙基淀粉自然沉降和羟乙基淀粉沉降后再用淋巴细胞分离液分离骨髓细胞。观察两种方法分离骨髓细胞后有核细胞、单个核细胞、血小板数。结果与结论:羟乙基淀粉两步法分离骨髓后有核细胞悬液中单个核细胞均值为89%,一步法为45%。两步法浓缩单个核细胞的百分率高于一步法(P<0.05);残留血小板数(中位数:73.00)低于一步法(中位数:367.50)(P<0.05)。结果表明,羟乙基淀粉和淋巴细胞分离液两步法分离骨髓细胞中单个核细胞和血小板的效果优于一步法。

BACKGROUND：It can improve the concentrated efficiency of mononuclear cell to use hydroxyethyl starch with lymphocyte separating medium.However,there is no report addressing the residual volume of platelets.OBJECTIVE：To detect the efficiency of isolating mononuclear cells and residual volume of platelets using hydroxyethyl starch by one step or two-step way.METHODS：Bone marrow was obtained from the samples of patients treated with adult autologous bone marrow karyocyte transplantation performed at the First Affiliated Hospital,Xinjiang Medical University,China.Bone marrow tissues acquired from patients were sedimentated by hydroxyethyl starch solution only,or followed by further centrifugation with lymphocyte separating medium.Karyocytes,mononuclear cells and platelet number by these two methods were observed.RESULTS AND CONCLUSION：The average percentage of mononuclear cells in two-step group（89%） was significantly higher than one-step group（45%）（P 0.05）.Moreover,the number of platelets was lower in two-step group（median：73.00） than one-step group（median：367.50）（P 0.05）.Results have suggested that the isolation efficiency of using hydroxyethyl starch solution by two-step way from human bone marrow was higher than one-step way.