当归多糖对体外造血微环境中肌卫星细胞增殖的影响(英文)

Effects of angelica polysaccharides on the proliferation of mouse skeletal muscle satellite cells in hematopoietic microenvironments in vitro

背景:肌卫星细胞是造血功能重建最有希望的种子细胞来源。当归多糖对造血干细胞和造血祖细胞的增殖分化存在显著的促进作用,也可以有效改变肌卫星细胞的生长特性。目的:观察当归多糖对不同培养条件下乳鼠骨骼肌卫星细胞增殖的影响。方法:分离小鼠肌卫星细胞,培养5d后采用α-肌动蛋白免疫细胞化学鉴定。将细胞接种于96孔板培养24h,使细胞同步化;将细胞分为空白对照组,骨髓基质细胞培养上清组,加入含50,100,200,300,400mg/L当归多糖的DMEM/F12培养基实验组及经50,100,200,300,400mg/L当归多糖干预后骨髓基质细胞条件培养基组。经实验处理后采用MTT法检测各组细胞的增殖活性。结果与结论:分离培养的骨骼肌卫星细胞呈α-肌动蛋白染色阳性,通过MTT法检测发现,经不同浓度当归多糖干预后的骨髓基质细胞条件培养基培养的各组肌卫星细胞增殖显著。且经当归多糖干预的骨髓基质细胞条件培养基可以有效改变肌卫星细胞的生长特性,并呈剂量依赖性。

BACKGROUND：It is hopeful that skeletal muscle satellite cells（SMSCs） can be served as seed cells for hematopoietic reconstitution.Angelica polysaccharides（APS） can not only promote hematopoietic stem cells and hematopoietic progenitor cells proliferation and differentiation,but also change the growth characteristics of SMSCs.OBJECTIVE：To investigate the effects of APS on the proliferation of mouse SMSCs in different culture environments.METHODS：SMSCs were procured by a modified method from new born mouse.The α-actin protein of the SMSCs was examined by immunohistochemistry at 5 days after culture.SMSCs were cultured and synchronized for 24 hours in the 96-well plate.After that,SMSCs were assigned into the blank control group,marrow stroma cell supernatant group,APS DMEM/F12 groups（contained 50,100,200,300,400 mg/L APS） and the marrow stroma cell conditioned medium（disposed by 50,100,200,300,400 mg/L APS in DMEM/F12）.The proliferation of SMSCs was determined by MTT.RESULTS AND CONCLUSION：The α-actin was positive in the cultured SMSCs.MTT results demonstrated that,SMSCs showed a proliferative property in the marrow stroma cell conditioned medium groups.Additionally,the marrow stroma cell conditioned medium can effectively alter growth characteristics of SMSCs in a dose-dependent manner.