孕期脉冲电磁场与子代大鼠海马神经干细胞增殖及巢蛋白的表达(英文)

Effects of prenatal pulsed electromagnetic fields on neural stem cell proliferation and nestin protein expression in the hippocampus of rat offspring

背景:电磁场能对机体产生影响,尤其对于神经系统。研究已发现了脉冲电磁场对神经干细胞的影响。目的:观察产前脉冲电磁场对子代大鼠海马神经干细胞增殖及巢蛋白表达的影响。方法:选用体质量240～260g SD雌性大鼠为母鼠,随机分为2组。对照组孕期不给任何干预;产前脉冲电磁场组于怀孕14～20d,给予脉冲电磁场刺激,3次/d,10min/次。于雌、雄仔鼠1月龄时每组随机各组6只行脑组织切片,应用免疫组织化学方法检测海马中Nestin蛋白和Brdu阳性细胞的表达。结果与结论:产前脉冲电磁场组雌雄子代海马Nestin蛋白和Brdu阳性细胞表达均较对照组多(P<0.001),且产前脉冲电磁场组雌性子代海马Nestin蛋白和Brdu阳性细胞表达较雄性子代的多,差异具有非常显著性意义(P<0.001),对照组雌雄子代之间差异无显著性意义(P>0.05)。结果表明,产前脉冲磁场能引起子代海马神经干细胞数增多及增殖能力增加,这可能是机体对产前脉冲电磁场所致脑损伤的代偿性反应。

BACKGROUND：Electromagnetic fields can cause changes of the body,especially the nervous system.Effect of pulsed electromagnetic fields（PEMFs） on neural stem cells has been detected.OBJECTIVE：To investigate the effect of prenatal pulsed electromagnetic fields（PPEMFs） on neural stem cell proliferation and nestin protein expression in the hippocampus of rat offspring.METHODS：Sprague Dawley female rats weighing 240-260 g were included and randomly divided into two groups：control and PPEMFs.Rats from the control group were given no interventions.Rats from the PPEMFs group were given PEMFs stress at gestational days 14-20.Each stress was given three times daily for 10 minutes.The male and female offspring rats were sacrificed at 1 month of age and their brains were sectioned to determine the expression of nestin protein and Brdu-positive cells in the hippocampus by immunohistochemistry.RESULTS：The expression of nestin-and Brdu-positive cells in the hippocampus of female and male PEMFS offspring were significantly higher compared with the control group（P 0.001）,and there was a significant difference between female and male offspring（P 0.001）.The nestin-and Brdu-positive cells in female offspring outnumbered those in male offspring（P 0.001）;however,there was no significant difference between female and male offspring in the control group（P 0.05）.CONCLUSION：PPEMFs can increase the number and proliferative capability of the neural stem cells in offspring.It may be a primary stage of the cascade reaction of the body to the brain damage caused by PPEMFs stress.