外科吻合用壳核结构磁性纳米钕铁硼的制备及其细胞毒性

Preparation and cytotoxicity of SiO_2/Nd-Fe-B core-shelled nanoparticles applied to surgical anastomosis

背景:磁力压榨式吻合器以其优越的性能受到越来越多的关注,但是其留置体内会对机体造成不良影响,可降解磁力吻合器有望解决此问题。目的:制备适于外科吻合用的硅胶表面修饰的壳核结构纳米钕铁硼(SiO2/Nd-Fe-B)磁性材料,并评价其细胞毒性。方法:高能球磨法制备纳米钕铁硼(Nd-Fe-B)材料,溶胶-凝胶法对其进行表面修饰。MTT法检测纳米SiO2/Nd-Fe-B材料的细胞毒性,纳米SiO2/Nd-Fe-B材料与L929细胞共培养,观察其在细胞内分布以及细胞器的改变,传代培养检测粒子代谢情况。结果与结论:成功制备出纳米SiO2/Nd-Fe-B材料,MTT法检测材料浸提液细胞毒性分级为1级,其吸光度值与空白对照组相比差异无显着性意义(P>0.05)。纳米SiO2/Nd-Fe-B材料可以通过胞吞方式进入细胞内,其在胞内分布于细胞浆中,TEM观察细胞线粒体轻微水肿,内质网扩张。传代培养发现3代内胞内纳米材料迅速减少。提示高能球磨法结合溶胶-凝胶法可以制备纳米SiO2/Nd-Fe-B材料,此材料细胞毒性小,符合植入人体生物材料的细胞毒性要求。纳米SiO2/Nd-Fe-B材料通过胞吞方式进入细胞内,对细胞器影响轻微,代谢迅速。

BACKGROUND： Magnetic compression device has acted increasing attention due to its superior performance, however, its retention in vivo would adversely affect the body, and biodegradable magnetic device is expected to solve this problem. OBJECTIVE： To product core-shelled SiO2/Nd-Fe-B nanoparticles which can be applied to surgical anastomosis and to evaluate their cytotoxicity. METHODS： The Nd-Fe-B nanoparticles were produced with high energy ball milling method, surface modification to the Nd-Fe-B nanoparticles was performed with sol-gel method. The cytoxicity was tested using MTT method. The core-shelled SiO2/Nd-Fe-B nanoparticles were co-cultured L929 cells, to observe the distribution of SiO2/Nd-Fe-B nanoparticles in cells and pathology of subcellular organelle, as well as the metabolism of SiO2/Nd-Fe-B nanoparticles in the subculturing cells. RESULTS AND CONCLUSION： The core-shelled SiO2/Nd-Fe-B nanoparticles were successfully produced, the result of MTT test indicated that the cytotoxicity degree of the extract of core-shelled SiO2/Nd-Fe-B nanoparticles was 1, without significant difference of absorbance value with blank control group （P 0.05）. SiO2/Nd-Fe-B nanoparticles could enter the cells though endocytosis, and distributed in the cytoplasm without nucleus, transmission electron microscopy found a slight swelling of mitochondria and expansion of endoplasmic reticulum. Subcultured cells which contained SiO2/Nd-Fe-B nanoparticles found that nanoparticles rapidly reduction during three subcultures. SiO2/Nd-Fe-B nanoparticles can be produced using high energy ball milling combined with sol-gel method. SiO2/Nd-Fe-B nanoparticles have mildly toxicity, and can meet the requirement of the national standard for medical implantation materials. SiO2/Nd-Fe-B nanoparticles could enter the cell through endocytosis, with slight influence on organelle and fast metabolism.