高级氧化法改性镍钛合金表面后变形链球菌及白假丝酵母菌的黏附能力

Influence of advanced oxidization modification on nickel-titanium alloy surface on adherence of streptococcus mutans and candida albicans

背景:目前国内外对于镍钛合金改性的方法多种多样,目的就是根据镍钛合金的临床使用要求,通过各种方法不断提高镍钛合金的生物安全性、生物相容性和抗腐蚀性,以期在临床上获得更为广泛的应用前景。目的:检测口腔医用镍钛合金高级氧化法改性前后,变形链球菌、白假丝酵母菌在材料表面黏附和定植的数量,分析高级氧化法改性对镍钛合金表面微生物黏附能力的影响。方法:将抛光组和改性组放入含有变形链球菌(streptococcus mutans,C型,ATCC25175)和白假丝酵母菌(candida albicans,ATCC76615)的培养基中培养,在24,48,72h3个时间点将试件取出,标本原液进行10倍稀释,取稀释液0.1mL接种在相应培养基中,48h后行菌落形成单位计数。结果与结论:随着时间推移,抛光组和改性组中微生物的黏附量均增高;在同一个时间点,改性组表面微生物的黏附量少于抛光组(P<0.01)。结果提示在唾液环境中,高级氧化法表面改性能够明显抑制镍钛合金表面对细菌的黏附能力。

BACKGROUND： There are various current methods for nickel-titanium alloy modification, with the aim to gain broader clinical application according to clinical use requirements for nickel titanium alloy, based on various methods of increasing biological safety, biocompatibility and corrosion resistance of nickel-titanium alloy. OBJECTIVE： To determine the adherence of streptococcus mutans and candida albicans on material surface and field planting number after advanced oxidization modification on nickel-titanium alloy, and to analyze the influence of advanced oxidization modification on the microbial adherence on alloy surface. METHODS： Polished and modified nickel-titanium groups were placed in culture mediums which contained streptococcus mutans （type C, ATCC25175） and candida albicans （ATCC7661）. After 24, 48 and 72 hours, the test pieces were taken out, the sample solution was diluted ten folds, 0.1 mL dilution solution was incubated in the culture medium, colony formation units were counted. RESULTS AND CONCLUSION： As time passed, the microbial adhesion levels were increased both in the polished groups and the modified groups. At the same time, the adhesion level in modified groups was significantly less than that in polished groups （P 0.01）. Advanced oxidation modification of nickel-titanium alloy in the oral environment can significantly inhibit the bacterial adhesion on the nickel-titanium alloy surface.