纳米晶羟基磷灰石胶原复合骨髓间充质干细胞修复骨缺损(英文)

Nano-hydroxyapatite/collagen composite co-cultured with bone marrow-derived mesenchymal stem cells for repair of bone defects

背景:临床上对大范围骨缺损还没有很有效的治疗手段,而纳米晶羟基磷灰石胶原复合材料与天然骨骼的结构类似,具有较好的生物相容性,可能为修复骨缺损提供新的途径。目的:观察纳米晶羟基磷灰石胶原材料复合骨髓间充质干细胞在修复骨缺损中的作用。方法:分离培养人骨髓间充质干细胞,与纳米晶羟基磷灰石胶原材料于体外联合培养;通过大体观察、组织学分析及电镜观察了解成骨情况,进一步临床应用于修复骨缺损。结果与结论:人骨髓间充质干细胞在体外可以大量扩增,复合细胞的材料植入骨缺损处后,X射线摄片动态观察可见骨缺损处连接良好。说明骨髓间充质干细胞具有成骨细胞作用,纳米晶羟基磷灰石胶原材料是一种很好的构建组织工程骨的支架材料。

BACKGROUND： There have been no effective means for clinical treatment of large regions of bone defects. Nano-hydroxyapatite/collagen （nHAC） composite would provide a new pathway for repair of bone defects owing to its similar structure to natural skeleton and better biocompatibility. OBJECTIVE： To investigate the role of nHAC composite co-cultured with bone marrow-derived mesenchymal stem cells （BMSCs） in repair of bone defects. METHODS： Following isolation and culture, human BMSCs were co-cultured with nHAC composite. Gross observation, histological analysis, and electron microscope observation were performed to analyze osteogenesis for repair of bone defects in the clinic. RESULTS AND CONCLUSION： Human nHAC could greatly proliferate in vitro. X-ray photography revealed that bone defects well healed after implantation of nHAC/BMSCs composite. These findings indicate that BMSCs exhibit osteogenic potential and nHAC is a satisfactory scaffold material for construction of tissue-engineered bone.