人类D19S40基因座在不同人种中的遗传多态性研究

Allele and genotype frequency distribution of D19S400 locus in several populations

采用ＰＣＲ技术分析中国汉族、德国人、斯洛伐克人和美国黑人群体Ｄ１９Ｓ４００基因座的遗传多态性及世界三大人种之间的差异。四个群体共调查了６２０人，发现了１１个等位基因，观察到４７种基因型。各群体观察杂合度为：０．７８～０．８８，个人识别机率为：０．９３８５０～０．９６６４。四个群体基因型频率分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡（Ｐ＞０．０５），三大人种（蒙古人种、高加索人种、美国黑人）之间Ｄ１９Ｓ４００基因座等位基因频率分布存在极显著差异（Ｐ＜０．０１）。

Using polymerase chain reaction and PAG electrophoresis,D19S400 Locus was investigated in the populations of Chinese,Germany,Slovakian,and American Black Population.620 samples collected from the four populations were tested and a total of 11 alleles (*7 to *17)and 47 genotypes were found.The heterozygosity was observed between 0.78 and 0.88,and the discriminating power was between 0.9385 and 0.9664.The distributions of genotypes in the four populations were in accordance with Hardy-Weinberg equilibrium.Significant differences were observed in the distribution of allele frequencies among the three major racial populations (Mongoloid,Caucasian,Negroid).According to the results obtained in this study,D19S400 locus is a robust STR for forensic and genetic purposes.