Sprouty2蛋白在肾透明细胞癌组织中的表达及意义

Expression and significance of Sprouty 2 protein in clear cell renal cell carcinoma

目的检测肾透明细胞癌及癌旁组织标本中Sprouty2蛋白的表达情况,分析其表达水平与肿瘤的临床进展及患者预后的相关性。方法采用免疫组化与Western blot方法对92例根治性肾切除肿瘤及癌旁组织标本以及8例非肿瘤肾组织中Sprouty2的表达进行检测,并结合临床病理学资料及预后进行统计学分析。结果肾癌中Sprouty2蛋白的表达较癌旁及非肿瘤肾组织中的表达显著减少,并且与肿瘤大小、有无转移、高分期、高分级有相关性(P均<0.01)。Kaplan-Meier生存分析显示Sprouty2低表达组与高表达组之间差异有统计学意义(P<0.01),单因素及多因素回归分析显示Sprouty2的表达水平可作为一个独立参数以评估肾癌患者的预后。结论 Sprouty2的低表达与肾癌的发生及进展相关,可能作为肾癌预测及预后评估的指标。

Objective To find out the association of Sprouty2 protein with the clinicopathologic features and clinical outcome in clear cell renal cell carcinoma（RCC）.Methods Samples were obtained from 92 patients treated with radical nephrectomy and 8 patients with nontumorous renal disease.Immunohistochemical staining and Western blot analysis were performed and a negative finding was defined as staining of 10% or less of the malignant cells.The degree of expression of Sprouty2 was compared with the clinicopathologic features.Results Sprouty2 expression was down-regulated significantly in RCC compared with tumor-free kidney,associated with tumor size（P0.01）,presence of metastasis（P0.01）,high pT stage（P0.01）,high-grade（P0.01） and low survival（P0.01）.Univariate（P0.01） and multivariate（P0.05） Cox regression analysis revealed that Sprouty2 expression is an independent prognostic parameter in renal cell carcinoma.Conclusions Inhibition of Sprouty2 is associated with progression and tumor recurrence.Sprouty2 may be a useful independent prognostic marker for this condition.