尿样中海洛因代谢物的测定及海洛因滥用的确认

Determination of metabolites of heroin in urine and discrimination of heroin abuse

用ＳＰＥ－ＧＣ－ＮＰＤ法建立了尿样中吗啡、６－单乙酰吗啡及可待因的定性分析方法，适用于海洛因滥用者的尿样分析。尿样中吗啡及可待因的最小检测限均为５０ｎｇ／ｍｌ。方法的相对标准偏差分别为：吗啡１１．３％（ｎ＝５），可待因１４．２％（ｎ＝５）。方法简便、灵敏、快速，１５ｍｉｎ可完成一例尿样的分析。研究了服用含可待因成分的复方甘草合剂后，尿样中的吗啡及可待因的峰面积比为０．４５７±０．１９７（Ｐ＝９９％）；统计了４０例明确滥用海洛因尿液的分析结果，吗啡与可待因的峰面积比为３．４６±０．８９４，Ｐ＝９９％。可作为判断海洛因滥用的依据。同时与免疫板法比较。

This article describes a sensitive method that detects morphine,6-monoacetylmorphine,morphine-3-glucuronide and codeine in urine for qualifying the abuse of heroin.The analytes were extracted by solid phase C18. The limits of detection (LOD) for morphine and codeine were 50ng/ml and 50ng/ml,respectively.The RSD of morphine and codeine were 11.3%(n=5),and 14.2%(n=5) respectively.For urine,it does not need to be hydrolyzed before extracted,and for all analytes,also need not to be derivated.The difference ratio of morphine and codeine in the chromatography can be used to discriminate between the abuse of heroin and the administration of compound liquorice mixture.