荷人膀胱癌严重联合免疫缺陷鼠自发性转移模型的建立

Establishment of human bladder cancer model with spontaneous metastasis in severe combined immume deficiency mice

目的建立荷人膀胱癌SCID鼠模型并观察其自发性转移情况。方法体外侵袭筛选膀胱癌细胞株T24,完成三次筛选的子代细胞记作T24-3;选取24只雌性SCID鼠,随机分为膀胱原位模型组和皮下异位模型组各12只,原位模型组采用酸预处理膀胱黏膜经尿道灌注3×106个T24-3细胞,同样剂量细胞注射接种于异位模型组鼠右后肢内侧皮下。通过成瘤性试验及鼠外周血中细胞角蛋白20(CK20)mRNA RT-PCR检测判定肿瘤模型及自发转移的发生。结果膀胱原位组64%成瘤,皮下组100%成瘤,肿瘤组织病理类型为移行细胞癌;且至6周膀胱原位组部分鼠出现肉眼可见多处远隔转移灶;不同周次部分鼠外周血中表达人CK20mRNA,至6周分别为5/11例和4/12例阳性表达。结论用经过侵袭筛选的高转移亚群T24-3细胞,行原位或异位种植可以成功制备荷瘤SCID鼠模型,并实现自发性转移。

Objective To develop a human bladder cancer model in SCID mice and investigate its metastatic biological features.Methods T24-3 cell line was isolated from human bladder transitional cell carcinoma T24 cell line by Boyden chamber system.Twenty-four female SCID mice were randomly divided into two groups.In group one,12 mice were implanted with 3×106 T24-3 cell on bladder mucosa,which were altered by mild acid pretreatment.In group two,12 mice were subcutaneously injected with T24-3.And then,the appearance of tumor and human CK20mRNA（＋） in mice peripheral blood by RT-PCR were adopted to investigate metastasis of tumor cells.Results 7 and 12 mice had pathological transitional cell carcinoma tumor in the orthotopic model group and the subcutaneous model group respeitively.Besides,there were macroscopic distant metastatic tumors in the orthotopic model group after 6 weeks.The expression of human CK20mRNA（＋） in mice peripheral blood by RT-PCR between the two groups was different,with 5/11 and 4/12 mice in the orthotopic model group and the subcutaneous model group.Conclusions Bladder tumor orthotopic or subcutaneous model in SCID mice with spontaneous metastasis can be successfully established by T24-3 cells.