糖尿病大鼠记忆功能改变及神经生长因子的影响

Changes of the memory and the therapeutic effects of nerve growth factor in diabetic rats

目的：观察糖尿病大鼠学习记忆功能的变化及外周投用神经生长因子（ＮＧＦ）对其的影响。方法：采用水迷宫试验及大脑海马ＣＡ１区神经元计数测定糖尿病成模后２个月，后肢肌肉注射ＮＧＦ２００μｇ·ｋｇ－１·ｄ－１，３０ｄ后的学习记忆功能改变，并与正常对照组（ＮＣ）组及糖尿病（ＤＭ）组比较。结果：ＤＭ组与ＮＧＦ治疗组相似，水迷宫试验游完全程的时间明显长于ＮＣ组；错误反应次数明显高于ＮＣ组，而海马神经元计数明显少于ＮＣ组，Ｐ均小于０．００１。结论：糖尿病损害大脑的学习记忆功能，外周投用ＮＧＦ对这种损害的治疗作用不明显。

Objective:To observe the changes of learning and memory function and the therapeutic effects of nerve growth factor (NGF) in diabetic rats.Methods: Two months after the diabetic model was induced, and nerve growth factor 200 μg·kg -1 ·d -1 . 30 d im was given for the NGF group. the faction of learning and memory were measured by the water maze testing and the hippocampal pyramidal cells were counted and compared with NC group and DM group. Results: Three months later, the time of completing the whole swimming journey and the numbers of error reaction were much higher in the DM group than in the NC group. The counts of hippocampal pyramidal cell in the DM group were much less than that in the NC group ( P <0 001). The parameters in the NGF group were similar to DM group. Conclusion: The cerebral function of learning and memory may be injured by diabetes, and peripheral administration of NGF shows not any improvement for such injury.