XT-4000i血液分析仪检测异型淋巴细胞的实验研究

Experimental study of atypical lymphocyte detecting by XT-4000i automatic routine hematology analyzer

目的通过XT-4000i血液分析仪白细胞分类检测通道(DIFF通道)检测高荧光淋巴细胞(highfluorescence lymphocytes,HFL)区域即HFL计数与人工镜检、EB病毒壳抗原(EBV-VCA)检测结果比较,探讨XT-4000i血液分析仪对异型淋巴细胞检出意义。方法 XT-4000i血液分析仪对230份标本采用DIFF通道检测HFL,与显微镜下计数异型淋巴细胞比较,并用血清作免疫酶标法检测EBV-VCA。结果 XT-4000i血液分析仪对异型淋巴细胞检出的假阳性率为13.6%,假阴性率为2%。结论 XT-4000i血液分析仪对异型淋巴细胞的检出有一定的有效性,对临床诊断效率的提高有一定帮助。

Objective To study the significance of atypical lymphocyte detection by the high fluorescence lymphocyte（HFL） area（HFL-count） from the XT-4000i hematology analyzer leucocyte differential（DIFF） channel,and compare the results of manual microscopy and epstein barr virus-viral capsid antigen（EBV-VCA） detection.Methods HFL-count was analyzed by DIFF channel in total 230 samples,and the results were compared with those of manual microscopy.Serum EBV-VCA detection based on the enzyme immunoassay.Results The false positive rate of atypical lymphocytes by XT-4000i hematology analyzer was 13.6%,and the false negative rate was 2%.Conclusions There is a certain validity for atypical lymphocyte detection by XT-4000i,and XT-4000i can improve the efficiency of clinical diagnosis.