三疣梭子蟹雌雄个体遗传差异的SRAP分析

SRAP analysis of genetic variation between female and male swimming crab (Portunus trituberculatus)

应用三疣梭子蟹SRAP-PCR优化体系,从88个SRAP引物组合中筛选出17个组合对三疣梭子蟹(Portunus trituberculatus)雌、雄个体遗传差异进行分析,共获得146个位点,雌性个体获得139个位点,多态位点比例为69.78;雄性个体检测到129个位点,多态位点比例达79.07。雌性和雄性的Nei's基因多样性分别为0.2315和0.2682,Shannon's信息指数为0.3478和0.4026,雌雄个体间的相似系数为0.5959～0.8417,遗传距离为0.1583～0.4041。用TFPGA软件绘制了个体间的聚类图。另外在5个SRAP选择扩增组合中发现了6个位点在雌雄个体间扩增差异较大,其中4个位点显性条带个体为雄性的比例为12.5%～20%;2个位点显性条带个体为雌性的比例为100%。

Using the SRAP-PCR optimization system for P.trituberculatus,this study applied sequence-related amplified polymorphism （SRAP） technique with 17 primer pairs screened out of 88 primer combinations to analyze the genetic variation of female and male populations.146 loci were obtained and the percentage of polymorphic loci was 69.78% in female population and 79.07% in male population.Nei’s gene diversity was 0.231 5 and 0.268 2,Shannon’s information index was 0.347 8 and 0.402 6.Genetic similarity index ranged from 0.595 9 to 0.841 7 and the genetic distance was 0.158 3 to 0.404 1 between female and male.A dendrogram was constructed using TFPGA.6 loci were identified from 5 primer combinations.Male genotype is dominant in 4 loci,whose ratio was 12.5% and 20% ,and female ratio was 100% in dominant individuals of 2 loci.