七彩神仙鱼同工酶表达的组织差异性分析

Tissue divergence of isoenzyme expression in Symphysodon spp.

运用聚丙烯酰胺垂直板凝胶电泳法,对七彩神仙鱼的8种组织(脑、皮肤、肌肉、肝脏、胰脏、鳃、眼球晶状体、心脏)中的9种酶类[酯酶(EST)、醇脱氢酶(ADH)、乳酸脱氢酶(LDH)、山梨醇脱氢酶(SDH)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、葡萄糖-6-磷酸脱氢酶(G-6-PD)和酪氨酸酶(COX)]进行了研究,并对其各组织中的同工酶位点及其酶谱表型进行了分析。试验中共检测到29个基因座位,分别由相应的等位基因决定。试验结果表明,七彩神仙鱼的9种同工酶系在各组织都显示出清晰稳定的酶谱,除CAT在七彩神仙鱼各组织和个体中表现一致以外,其它8种同工酶都存在明显的组织差异性和一定的个体差异,组织差异与其生理功能相一致。肝脏和胰脏中的同工酶位点信息丰富,肝脏中的EST和ADH表达活跃;肝脏和眼中的LDH存在C位点,且眼球晶状体中的C位点存在和A、B位点杂合的情况。

EST,ADH,LDH,SDH,POD,SOD,CAT,G-6-PD and COX isoenzymes in eight tissues （i.e.brain,skin,muscle,liver,pancreas,gill,eye lens and heart） of Symphysodon spp.were studied by PAGE.And the locus and electrophoretogram in every tissue were analyzed.Total 29 gene loci were detected with corresponding alleles.All the 9 isoenzyme systems in every tissue have distinct and stable electrophoretograms.Besides CAT,the electrophoretograms of the other isoenzymes varied from different tissues or individuals.The isoenzymes and their activity were consistent with physiological functions.The liver and pancrease were abundant with locus.The EST and ADH in the liver have high activities.There existed Cloci in the LDH of livers and eyes,and the C-loci of the eye lens were heterozygous with the A-loci and Bloci.